In time-resolved crystallography (TRX), x-ray diffraction by crystals is utilized to examine in real time the structures of proteins as they are changing and therefore improve understanding of their function and gain important structural information on specific protein targets.
Five-dimensional (5-D) crystallography is a specific form of the TRX technique where, in addition to space and time, temperature is a variable as well. It allows complete characterization of all features of a chemical reaction, including the structure of its intermediate compounds, as well as the reaction kinetics, and barriers of activation between the intermediates. It provides an essential and direct link between the structural changes and energy changes in the chemical reaction.
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