In Victoria, 200 kilometres west of Melbourne, over 260 trees sacred to the Djap Wurrung peoples - including a traditional birthing tree - have been flagged to be bulldozed in preparation for a $42 million upgrade to the Western Highway.
The plans will duplicate 12.5 kilometres of the highway from Buangor to Ararat and reduce travel time on the route by an estimated two minutes.
The Victorian government subsequently altered the plans for the duplication route in February but campaigners said that it would still impact on culturally significant trees.
Demonstrators set up three camps on the land last month in an attempt to stop roadworks.
An emergency application was also lodged to protect the trees under the Aboriginal and Torres Strait Islander Heritage Protection Act.
“These beautiful trees include an 800-year-old tree that has seen over 50 generations born inside of a hollow in her trunk and a 350-year-old directions tree that has been shaped and resembles a woman,” the Djab Wurrung Embassy website says.
“This area is part of the song line, the series of scared trees and artefacts we find here regularly prove its significance.”