One interesting thing that can happen in long running media is that the general cultural background can shift under the work, recontextualizing it as it is being written.
I'm specifically thinking of the Order of the Stick, a Dungeons and Dragons themed webcomic that started in 2003 with the titular party of adventurers going through a dungeon.
From left to right, we have Belkar Bitterleaf the halfling ranger, Vaarsuvius the Elf Wizard, Elan the Human Bard, Haley Starshine the Human Rogue, Durkon Thundershield the Dwarf Cleric, and Roy Greenhilt the Human Fighter.
The comic takes place in a fantasy setting that knowingly runs off the rules of Dungeons and Dragons third edition. Characters talk about rolls and bonuses and intentionally take levels in various classes. At the start, the comic was a pretty basic gag comic about the D&D rules, basic fantasy/adventure tropes, ect.
In the 20 years the comic has been running, it has updated about 1300 times, not counting bonus strips exclusively made for the printed version, and several print (or PDF) only side and prequel stories. It has also dramatically grown from it's roots, the art has improved while keeping the same general aesthetic, and the gag-a-day comic has become a sweeping fantasy epic. The characters have grown beyond their initial bits (Belkar is a Murderhobo, Elan is stupid, Haley is greedy, ect), and it's genuinely up there as one of my favorite stories.
But anyway, let's talk about Vaarsuvius. If you look at the above art, You'll notice that the characters tend to have three types of body shapes: Rectangles for Roy, Belkar, and Elan, feminine curves for Haley, and Robes for Vaarsuvius. This presentation is a pretty consistent signifier of gender and/or somebody wearing robes.
Early on, part of Vaarsuvius's running gag became their ambiguous gender. At the time, it was a fairly common joke in fantasy to talk about how Elven men had androgynous or "Girly" appearances, so V was part of that. Instead of a singular pronoun, characters would generally just abbreviate Vaarsuvius's name as "V", and whenever the narrative would have naturally provided some indication of gender one way or another, V would resolve the situation without providing any such indication. For example, an early gag has the characters seeking out a set of modern style bathrooms in the dungeon. When they find them, V says that their "More Efficient elven biology" means they don't have to go yet, so they wait outside while the boys go into the Men's room and Haley waits in the inevitable long line at the women's. When Vaarsuvius reveals that they are married, they use the term "Spouse" to refer to their partner, when we see their children, the children are clearly adopted (V and their partner both have pale skin, their children have darker skin) and refer to Vaarsuvius as "Parent". Vaarsuvius themselves seems to have trouble identifying other people by gender. Characters outside the central cast might refer to Vaarsuvius as "He" or "She", but doing so was always shedding light on that character's perspective, rather than saying anything about Vaarsuvius.
The assumption behind the gag is that Vaarsuvius must be either male or female, and the joke is that the narrative/Vaarsuvius themselves keeps finding ways to avoid "Revealing" their gender. Fan wikis and official books list Vaarsuvius's gender as "Ambigious" and on the forum there used to be a regular, multi-part thread dedicated to debatings Vaarsuvius's gender, even after the author declared that it would "never be revealed".
Anyway, going back to the start, it's 2023, and something shifted at some point, both in the comic and in the general cultural background. The jokes about V's gender kind of fell off, not just because the gag got played out, but because the basic assumption behind it simply doesn't work anymore. Everybody knows that Nonbinary people exist. There's no point in the comic where Vaarsuvius switches from being "Ambigiously Gendered" to Nonbinary, in fact, the entire comic reads just fine if you read Vaarsuvius as male or female and just not caring enough to clarify their gender to anybody and at some point other characters just stop thinking about it. But it's interesting to see how a character trait that was once included in even the most basic character descriptions (Varsuvius: Elven Wizard. Arrogant, Intelligent. Ambigiously gendered) just kind of got washed away by a rising tide of cultural nuance towards gender.
Also go read OOTS, it's pretty great.
